MersV1, Main, Exploration, bibRecord, 004C28

Generation of a neutralization-resistant variant of HIV-1 is due to selection for a point mutation in the envelope gene

Identifieur interne : 004C28 ( Main/Exploration ); précédent : 004C27; suivant : 004C29

Generation of a neutralization-resistant variant of HIV-1 is due to selection for a point mutation in the envelope gene

Auteurs : Marvin S. Reitz Jr. [États-Unis] ; Carolyn Wilson [États-Unis] ; Christine Naugle [États-Unis] ; Robert C. Gallo [États-Unis] ; Marjorie Robert-Guroff [États-Unis]

Source :

Cell [ 0092-8674 ] ; 1988.

RBID : ISTEX:4D41DF71F903FD557B4FAD2D67102F440076D534

English descriptors

Teeft :
- Acid substitution, Active clone, Amino, Amino acid sequence, Amino acids, Antiserum, Assay, Clone, Complete medium, Complete nucleotide sequence, Continuous presence, Control viruses, Cysteine residues, Differential hybridization, Dimarzo veronese, Disease progression, Envelope glycoprotein, Envelope proteins, Epitope, Experimental procedures, Fragment, Gallo, Genetic variation, Genomic diversity, Genomic library, Glycoprotein, Human serum, Human virus type, Hxb2d, Hxb2d clone, Hxb2d sequence, Hxb2d virus, Immune selection, Immunodeficiency syndrome, Lambda, Lambda clone, Lambda phage, Large envelope glycoprotein, Large envelope protein, Molecular clone, Monoclonal antibodies, Monoclonal antibody, Mxb2, Mxb2 recombinants, Neutralizability, Neutralization, Neutralization assays, Neutralization behavior, Neutralization resistance, Neutralizing, Neutralizing antibodies, Neutralizing antibody, Neutralizing antisera, Neutralizing antiserum, Neutralizing epitope, Normal serum, Parental, Parental virus, Parental virus population, Point mutation, Recombinant, Recombinant virus, Recombinant viruses, Resistant variant, Restriction enzyme analyses, Same antiserum, Second clone, Second neutralizing serum, Secondary structure, Sstl, Sstl fragment, Sstl site, Substitution, Tissue culture fluids, Transmembrane, Transmembrane envelope protein, Transmembrane protein, Variant, Variant virus, Viral, Virol, Virus, Virus population, Visna virus, Xhol site.

Abstract

Abstract: Transmission and growth of HIV-1 produced from the biologically active clone HTLV-III/HXB2D in the constant presence of a neutralizing antiserum yielded a viral population specifically resistant to neutralization by the same antiserum. Molecular clones MX-1 and -2, containing the entire envelope gene, were obtained from cultures of the resistant variant. The coding regions for the large envelope protein and most of the transmembrane envelope protein of two such clones were substituted for the homologous segment of HXB2D. Infectious viruses from these constructs were also specifically resistant to neutralization by the selecting antiserum. The exchanged fragment contained only one base change, resulting in an Ala→Thr replacement at position 582. When this substitution was introduced into HXB2D it conferred the resistant phenotype. Thus, small differences may be selected for in vivo by the host immune response and result in relatively large differences in susceptibility of the virus to such a response.

Url:

https://api.istex.fr/ark:/67375/6H6-5G0JMMW2-8/fulltext.pdf

DOI: 10.1016/0092-8674(88)90179-1

Affiliations:

États-Unis

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Le document en format XML

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<term>Clone</term>
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<term>Complete nucleotide sequence</term>
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<term>Experimental procedures</term>
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<term>Genomic library</term>
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<term>Hxb2d clone</term>
<term>Hxb2d sequence</term>
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<term>Immunodeficiency syndrome</term>
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<term>Lambda phage</term>
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<term>Large envelope protein</term>
<term>Molecular clone</term>
<term>Monoclonal antibodies</term>
<term>Monoclonal antibody</term>
<term>Mxb2</term>
<term>Mxb2 recombinants</term>
<term>Neutralizability</term>
<term>Neutralization</term>
<term>Neutralization assays</term>
<term>Neutralization behavior</term>
<term>Neutralization resistance</term>
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<term>Neutralizing antibodies</term>
<term>Neutralizing antibody</term>
<term>Neutralizing antisera</term>
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<term>Neutralizing epitope</term>
<term>Normal serum</term>
<term>Parental</term>
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<term>Parental virus population</term>
<term>Point mutation</term>
<term>Recombinant</term>
<term>Recombinant virus</term>
<term>Recombinant viruses</term>
<term>Resistant variant</term>
<term>Restriction enzyme analyses</term>
<term>Same antiserum</term>
<term>Second clone</term>
<term>Second neutralizing serum</term>
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<term>Sstl fragment</term>
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<term>Tissue culture fluids</term>
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<term>Variant virus</term>
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<front><div type="abstract" xml:lang="en">Abstract: Transmission and growth of HIV-1 produced from the biologically active clone HTLV-III/HXB2D in the constant presence of a neutralizing antiserum yielded a viral population specifically resistant to neutralization by the same antiserum. Molecular clones MX-1 and -2, containing the entire envelope gene, were obtained from cultures of the resistant variant. The coding regions for the large envelope protein and most of the transmembrane envelope protein of two such clones were substituted for the homologous segment of HXB2D. Infectious viruses from these constructs were also specifically resistant to neutralization by the selecting antiserum. The exchanged fragment contained only one base change, resulting in an Ala→Thr replacement at position 582. When this substitution was introduced into HXB2D it conferred the resistant phenotype. Thus, small differences may be selected for in vivo by the host immune response and result in relatively large differences in susceptibility of the virus to such a response.</div>
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Generation of a neutralization-resistant variant of HIV-1 is due to selection for a point mutation in the envelope gene

Generation of a neutralization-resistant variant of HIV-1 is due to selection for a point mutation in the envelope gene

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri